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CytoSolve® reveals how Hyaluronic Acid affects TWO molecular pathways involved in Joint Health. Short video above. Full analysis:

Transcript Below.

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Good evening, everyone. It’s Dr. Shiva Ayyadurai.

Welcome all of you, we’re going to talk about the joint health system in your body. And as a part of that joint health system, today, we’re going to look at the system of hyaluronic acid and how it affects your body. So let’s go to hyaluronic acid.

So hyaluronic acid, what is it, it’s what’s called a disaccharide. Yesterday, we looked at conjoint was a polysaccharide. And it’s a, it’s a joint health supplement.

And you can see the connection of this, this looks a lot like a sugar, it’s got D glucuronic acid and acetyl D glucosamine. So here’s an acetyl D glucosamine right here. And here’s glucuronic acid right here.

So these two structures and SC galactosemia amine and D glucuronic acid make hyaluronic acid. So it’s really a naturally occurring. What’s a polymer is is multiple molecules that are connecting.

Now this is in both prokaryotic as well as eukaryotic cells, and its key role is in cushioning and lubricating the body. It’s clinically shown to protect cartilage. So that’s what hyaluronic acid is.

It protects your cartilage. It’s clinically shown to do that. But it’s really the cushioning aspect of what works and it’s found in both prokaryotic and eukaryotic cells.

So where do you get hyaluronic acid, these are some interesting areas. First of all, you can get it in bone broth, there’s a lot of people really supporting bone broth, one areas, bone broth, the other is organ meats, fish eyeballs, some cultures, as you may know, or you may not know are very much into in when I was in Singapore or dealt or in even in India, one of the most prized elements. If you eat fish, as you eat the entire fish out is called fish at soup and you eat everything because the fish head is known to have lots of nutrients.

In many cultures, you make traditional cultures, you make bone broth soup, right, you take bone, most cultures, you put a big bone in and you cook it very slowly. So the bone broth has a lot of hyaluronic acid. That’s the other piece.

These are the sources of it. So what are here’s some other sources. So remember I said prokaryotic and eukaryotic.

So this is a eukaryotic, the animal cells and prokaryotic. So unlike conda Royton, where you can only get an animal liquid hyaluronic acid, you can also get it in root vegetables, soy based foods, citrus fruits and leafy greens. You want to take note of that you’re getting it in the meat.

So those of people who are meat eaters, you’re also getting it in a vegetable sources. So multiple sources. So what are the biological effects of hyaluronic acid? What are those biological effects, one of the biological effects that hydrates your tissue.

This is what you’ll see this in a lot of skincare products, it lubricates your tissue, it provides structural integrity in cells and tissue. So what is structural integrity, if you if you understand transport, conversion storage, when we teach this to people in our course in our system, structural integrity, it’s the infrastructure. So if you think about the highway system in an organization, if you think about the cell membranes, if you think about the structure of your home, hyaluronic acid provides structural integrity.

It’s really the infrastructure part very valuable for that it provides and it’s a large polymer. It’s about 1000 kilodaltons. It’s a anti angiogenic and it’s an immunosuppressive What does anti angiogenic mean angiogenesis is a process that occurs knock on wood, no one gets cancer here, but when a tumor lands and it starts to proliferate, it starts putting out his own vasculature, which means its own blood supply lines.

That’s called angiogenesis. So hyaluronic acid, as the research shows, the large polymers are anti angiogenic, the small polymer. So there’s two sets are pro inflammatory pro angiogenic.

So there’s two types of polymers here. So the health benefits are it improves cartilage regeneration. And this is what our research has shown from looking at the literature, it improves skin hydration, promotes wound healing, it regulates skin aging, and it facilitates targeted drug delivery for tumor treatment.

What do I mean by that? What that means is a lot of the drug companies are incorporating hyaluronic acid because it can deliver antitumor drug to its place and make basically make things much more bioavailable. So even the drug companies use are interested in hyaluronic acid to facilitate targeted delivery. Let’s talk about hyaluronic acid.

So when you look at hyaluronic acid is quite well researched everyone see this nearly 40,000 papers written on hyaluronic acid 39,944 Over the last 82 years of medical research, and 1695 clinical trials. So just think about that. That’s a ton.

So if we look at this, what do we do at cytosol. We take all of that research, and then we drive it through a bio informatics process. So we’re looking at that research for identifying the key papers or relevant ones, the ones that had a lot of citations, references, etc.

And then out of that we extract out right here the molecular mechanisms which we’re going to walk you through and then we create an architecture in this case, we’re not looking at hyaluronic acid, it’s all different functions. This is across all different areas hyaluronic acid PRI for skincare, a whole bunch of things. We’re looking at the ones on the molecular system of joint health, and then we integrate all of these and then right now we’re publishing it directly To you, I wanted to let you know that we use the technology here.

We’ve helped many, many companies over the last 16 years, a lot of smart, innovative companies. But we decided with all the mathematical models we’ve created, why don’t we try to use this to compute the best product we could think of from the science out there for reducing pain and inflammation, pain and discomfort, and that resulted in us Creating m v 25. Using cytosol.

We’re gonna have more products that are going to be coming. But let me just show you what MB 25 is about for those of you who haven’t heard about it, but this is using cytosol in a beneficial way not to just do research, but find combination therapies, I am firebrand, my hands would cramp up so that I couldn’t hold cards or knit or crochet. And they would go like that, not have to use this.

When I played cards with my grandkids and I started taking that MV 25. After bed, I was able to hold cards in my hand, very, very little cramping hardly at all anymore. MV 25 Hi, my name is Sandy.

I’m a Taekwondo instructor, I tore my ACL during Taekwondo, I had a lot of pain and limited mobility. I’ve been taking the MV 25 for about six months. Now, after the first week, I noticed the big difference after the second week, almost literally no pain.

My name is Jeremy and I suffer from a lower back problem hurt my back at work years ago. And I could go to the chiropractor and do all kinds of different things. And nothing seems to help.

And I decided to try MV 25, I didn’t notice a difference immediately. But within a few days, that pain went away, and it stayed away, I’ve continued to take it. And even when I do things that I shouldn’t do, it seems to go away a lot quicker than I ever did before.

It’s clean food certified, it’s made in the US, if you go to big Right on the shop, you’ll click there. Or you can go right to MB 25 dot life either way, and then from there, you can click on the bottle and you can order.

If you buy six bottles, you get six bottles for free, please take advantage of it. Because first of all, it’s going to help you it’s going to help our movement. And it really supports the fact that we want to take science based approaches to natural products.

So we were able to take the systems approach to understanding joint health or human knee osteoarthritis. And one of the most important things that I’m very proud of was the research that we discovered, which I’m going to share with you very quickly is all the molecular mechanisms of joint health osteoarthritis, we just didn’t publish it in a little journal only for the dweebs to read or the finite set of scientists, we published it in an open source open science, so I’m going to share that with you. So if you go to cytosol.

com/in, I’ll show this to a knee osteoarthritis, you will literally go there right now I’m going to look go live. And this is the website, everyone’s welcome to go to assume everyone can see it here. And we published this for the world it was done with I want to thank our colleagues at University Health Program at the Division of orthopedics in Toronto, Ontario, as well as the cytosol biology systems biology program.

And by the way, there’s a white paper here where anyone can read it’s free open science, you can actually read all the stuff that we did, how we did all this, I encourage you guys to all go enjoy this. But for those who are not super science nerds, you can just use this diagram so you can explore the entire joint, we’re looking at the human knee joint, which is got the seven different tissues osteophytes fat pad meniscus subchondral bone, if you click on subchondral bone, it gives you the different cells that make it like osteoblast. And you go to Ostia blood, you can find the different ligands, right, and you can find the different cytokines and you can really look at TNF alpha, you can literally traverse all of these to understand how all of you know just this one little tissue when we look at cartilage, which is what we want to look at here.

Cartilage has a particular so cartilage has a cell’s cartilage is a tissue made up of cells called Condor sites, Condor sites are selling on that cell as many receptors and different molecules called lie hands can land on it, and one of them is called cytokines. Now here, if you look at il one beta, which is an inflammatory cytokine, I’m gonna explore that you can go look at all different kinds of different molecules that il one beta which is an inflammatory, cytokine upregulates. Those are the molecules in green.

And by the way, this was our Herculean effort. So if you look at Cox two Cox two as an inflammatory effect, and then you can go explore this and you can see here’s Iowan beta, it creates Cox two and you can see, hey, look at core two sets, which are very powerful mushroom quarter steps, literally prevent Iowan beta. Very cool, you can go back here and you can say I want to look at something else.

I want to look at human knee, I want to look at maybe something beyond the human knee architecture. I want to make the screws over here right? I want to go back and I want to look at a different line again here. Maybe I want to go look at how it up regulates another inflammatory cytokine call.

This is one I’d like to show off here because it’s pretty cool MMP 13 Which actually degenerates cartilage it has a catabolic effect. And when you go in through here, you can find out all the different molecules. So if you can see, here’s I one beta, il one beta gives rise to MMP 13.

And look at all these amazing nutrients that can inhibit it. So here’s ginseng, as I talked about, if you click on ginseng, you go right to the paper that has it. So literally, what we’ve done here is we’ve mapped out every molecular pathway of joint health.

And this has not been done. And more importantly, we’ve made this accessible to the world. We’re not and this is a way my view is part of the thing I think the world needs is we need citizen science, we need systems science.

So every one of you please take advantage of this. But this was about a three year research effort. So that’s what we’ve done here, we’ve created a way that all of you can become citizen scientists.

That’s really when we look at this whole mapping we did we want to look at how hyaluronic acid affects this mapping. We now let me go back here, we now wanted to look at the particular molecular pathways that affect joint health relative to hyaluronic acid. So getting back here, so that was all the research we did for about many years to put all that together.

So once you understand that, let’s go here. So what are the mechanisms? Now we’ve talked about this before, these are the four major mechanisms involved in joint health, okay, which I’ve talked about before inflammation, right, you get in for pain and inflammation, oxidative stress, cartilage degeneration, cartilage regeneration, these are the four main there’s many others involved in joint health. And these are the different molecular mechanisms which we’ve mathematically modeled with cytosol inflammation, oxidative stress, cartilage, degeneration, cartilage regeneration, and we’ve mathematically modeled this and for each one of these mechanisms, there are different biomarkers.

So if you have high levels of PG to inflammation is going on. If you have high levels of RLS which means reactive oxygen species that means you have the aspect of that means you got oxidative stress your body is undergoing rusting. Cartilage degeneration comes from that MMP 13.

cartilage regeneration is when you get collagen to development. So when you really think about it, there are four major mechanisms that you want to assess how does this ingredient affect any one of those forms? What you’re going to find is not all ingredients work for everything. This is why this deep dive analysis really helps you now when you’re going to shop for supplement this gives you like a grid what is hyaluronic acid really good for yesterday day before we talked about how MSM is good for oxidative stress we talked about yesterday, how can writing is good for cartilage regeneration, right? It wasn’t that great for inflammation, what is hyaluronic acid good for? So that’s what we want to find out.

So we find out hyaluronic acid on joint number one, the bio informatics analysis, let me just be clear at cytosol. We want to give you a holistic systems picture. So first, we looked at all the body of literature and what that says that’s called bioinformatics.

Then we take all the molecular pathways, and we mathematically model it in cytosol. And that will give us even like a deeper microscopic understanding. And that to us gives us a much deeper idea.

So you don’t just want to believe what this person says you want to combine it and further combine it with the computational approach. So if we take the bioinformatics approach our discovery showed from the bioinformatics is hyaluronic acid affects two of the joint health mechanisms cartilage degradation and cartilage regeneration so really affects these two, it doesn’t affect all four, it affects two, according to the bioinformatics research. So again, to be clear, it’s not going to help you with inflammation.

It’s not going to help you with oxidative stress. But our research shows it’s going to have profound effects, at least from the bioinformatics on degradation and regeneration. So hyaluronic acid increases cartilage regeneration, and it reduces cartilage degradation.

And so this is how it does it right. So it supports cartilage regeneration, and it stops cartilage regeneration. By the way, when you look at these drawings, there’s what I want to mention to all of you is the green arrow means it’s supportive of something the red thing with a hammer at the end.

This is again in biology or biochemistry means it stops something it’s inhibit inhibit something the other is it accentuates it up regulate something. So I’ll be doing a lot of those diagrams, you’ll see many of the talks will give and you by the way you can apply this, you can think about this, you can apply this tip, there are certain things that promote true good scientific method, scientific consensus stops true, there are certain things that promote health right, there are certain things that stop health certain things that support freedom, the First Amendment and the Second Amendment support freedom, censorship stops freedom. So you can take a systems approach.

What we see here is that hyaluronic acid as these two effects, and these are the two mechanisms of it. So now let’s talk about the health benefits. Again, from bioinformatics standpoint, how does hyaluronic acid stop current or support or stop cartilage degeneration? So cartilage engenders right we know il one beta, which is an inflammatory cytokine is a hallmark of joint disease.

So if you were to go to your doctor and you say, hey, look, can I have my Iowan beta levels checked? It’s a marker, it’ll tell you, you know, where you are in the spectrum for joint health joint disease, so aisle one beta, a very good marker, so if you have Iowan beta a lot in your blood, it’s Going to promote the production through this other such what it promotes p 38. Map k is going to give you MPP 13, which is going to cause cartilage degeneration. You don’t want this guess what hyaluronic acid does, boom, it stops us it stops il one beta from up regulating this to get this pathway.

So the downregulation MMP 13 right here by hyaluronic acid prevents cartilage degradation. That’s what the bioinformatics work does. But hyaluronic acid also supports cartilage regeneration hyaluronic acid activates aka T enzyme which supports collagen to which supports cartilage regeneration.

So let me just make that very clear. collagen to is a thing that you want your body to produce, right? If you’re doing you don’t want that to be degraded to collagen to production is supported by hyaluronic acid. So hyaluronic acid is a one two punch, it creates a K T which increase collagen to production.

And it stops Iowan beta going to MPP 13, which stops cartilage degradation. So it’s that’s what I mean by the one two punch. There you go.

So the key point here is that is a bioinformatics that’s from taking all the literature. Now what I’m going to do is we use the cytosol the other part of the technology to mathematically model the cartilage degradation and regeneration functions. And then we’re going to actually do experiments where we drop a certain amount of hyaluronic acid to see if it lowers MMP 13, right, because that’s the thing that’s involved in cartilage degradation.

And on the other hand, does it increase collagen too? So that’s what we did in our computational experiments, right? Again, no animals were killed in this research. So MMP 13 which is the biomarker of cartilage degradation. So we did experimental conditions we did dosage of 2.

5 to five milligrams per day over two days. So look at this, this is pretty cool. So you have a high level of you have 556.

1 level of MMP 13 And it brings it down doesn’t bring it down massively, but it definitely does bring it down. If you look at the scales here, so under controlled conditions MMP 13 where we have 456 Without hyaluronic acid increased supplementation showed a decreasing trend towards lowering MMP 13 And this is only for two days as you do it will probably go lower and lower hyaluronic acid has a low to moderate effect on cartilage degradation at the dosage study. So we only studied that this dosage.

And there you see the trend is it definitely reduces cartilage degradation. The next thing we did was we looked at college into production we started with around 1500 This goes up by nearly 20% Right 2015 To 20% massive improvement and this is only at about 2.5 to 10 milligrams per day.

So under control condition, the college had two values were 1520 right here that’s controlled this way are normal and with supplementation, it has a very nice effect at about 100 milligram dosage ironic acid and beyond that it doesn’t seem to we could go much higher, which we didn’t do which we can do. So the bottom line is what we’re finding is hyaluronic acid lowers cartilage degeneration, it gets rid of that and it increases collagen too. And we in fact if we do it as a heatmap so doesn’t affect these two pathways.

It affects somewhat nominally cartilage degradation but it definitely affects cartilage regeneration. So what is hyaluronic acid with dosa Jing for wound healing that came out in 2019 from Schneider at all is you can get it as a cream about point 2% cream you can go get this for wound healing for osteoarthritis Tyler at all says you can get to milliliter point 8% Highland GF which is 2016 milligrams solution you can take it every three days. So this is for osteoarthritis.

You can take it as a solution or you can get as a cream if you’re looking at wound healing because this is really supports the cartilage regeneration of tissue regeneration, but this is what the literature says right about two milliliters point 8% Remember Hyaluronic Jia 20 and 16 milligrams solution this was done in 2005 research every three days, so once every three days. So in conclusion, what we found out is that bioinformatics the literature says hyaluronic acid helps to reduce cartilage degradation improves cartilage regeneration, and it’s clinically proven improved joint out skin hydration, accelerate wound healing the site of soft computational analysis one step even deeper, says hilar Allah rock on is significantly effective for cartilage regeneration, very powerful anti aging, hyaluronic acid is low to moderately effective for cartilage degradation. So conjoining is very good.

So imagine combining this with con right we can do a whole nother research on that. So that’s what we found. Thank you, everyone.

Have a good evening. Hope you have a good dinner. I’m gonna go grab some right now.

Thank you

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