CytoSolve® reveals that GLUCOSAMINE affects 3 molecular pathways involved in JOINT HEALTH. Short video above. Full analysis: https://vashiva.com/how-glucosamine-affects-joint-health-a-cytosolve-systems-biology-analysis/
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ROUGH TRANSCRIPT (Auto-Generated)
Good evening, everyone is Dr. Shiva Ayyadurai. So this is glucosamine.
And we’re gonna talk about joint health, we’re gonna take a molecular systems biology approach using CytoSolve. The technology that I created for my PhD work out of MIT, and which we’ve been working on for 16 years. CytoSolve was a technology.
It’s a revolutionary technology that allows us to use a computer to eliminate the need for animal testing. So we can model complex biological phenomenon using the computer. And I’ll talk more about that, but we use CytoSolve.
Now, as a public service, we’ve used it to make money where we help companies who are trying to figure out what products did create, does it work or not, and our goals, we don’t need animal testing, I have a couple of dogs. In fact, the FDA even argues that you’re going to test something on humans, where you’ve been wasting time on dogs or animals that are etc, and causing them cruelty. It’s a whole nother discussion, but CytoSolve aims to eliminate that need.
And we’ve created that technology to do that. But this is really a molecular systems biology approach. So when you look at glucosamine CytoSolve, helps us do a number of things.
Look at all the articles on glucosamine nearly as much as hyaluronic acid is about 36,000 or as of right now 36,275 research articles, and that’s over 132 years of medical research 410 clinical trials, that’s a lot of information out there, and glucosamine. So if I were to tell you that you got to read all those papers and figure out what they say, and you can’t cherry pick the ones you like, you got to go through them. How would you do that.
So what happens in science today, people go cherry pick, and if they have a political agenda, but with CytoSolve, we have an approach where we can take those articles, and I’m going to share that with you, we have a very powerful way to take a methodological approach that allows us to organize those articles, and then extract out the molecular mechanisms relative to a particular system today system that we’re interested in, we’re interested in the system today of joint health too, if you’re interested in cardiovascular health, it would be a different way you organize the molecular system. So we’re looking at glucosamine relative to joint health. So when you look at this diagram, we can literally go through and we can organize that.
And then we can do mathematical simulations. So first, we organize the content. And we’ll get some indication that’s called the bioinformatics approach.
Now, having said that, I want to now go a little bit deeper into CytoSolve. Because I want you to understand the power of this technology. So we have all those 30,000 papers.
So aside is how we’re able to do what’s called two types of analysis. The first analysis is called when we’re bringing it down the funnel, the bioinformatics analysis, imagine having to read all that and really trying to figure out what’s the distilled facts, then we can do another piece called the computational analysis, which means we understand the molecular mechanisms, then we can go deeper to understand mathematically using CytoSolve to model all those molecular reactions. That’s what’s really cool about CytoSolve.
And then seeing what happens when we drop glucosamine into those molecular reactions. What will it actually do for that specific set of reactions in this case, are the reactions we’re interested in is joined town. So let’s go back here.
So CytoSolve, you can go to CytoSolve.com, if you want to learn more about it, but it came out of my PhD work. And one of the problems I saw in the pharmaceutical industry, which is really failing, and you’ll see why is the way they build a drug, by the way drug is not something that occurs in nature, they do test tube testing is called in vitro, if you want to impress your friends, in vivo means in the animal, they do about seven years of this, they kill a bunch of animals, they test the compound, they say, Oh, we think it’s doing something.
So they’ll give actually cause cartilage degeneration and animal give the compound so you’d affects it, then if they have gone through this, then they’ll apply to the FDA. And they’ll try to get the allowance to go do human trials, phase one, phase two, phase three. Anyway, this takes around 13 years, $5 billion, and less than 20% of the stuff entering phase one is even successful, very, very costly process.
And this is why pharmaceutical companies are looking to a new avenue of making money. This is why and you’ll see right, here’s the economics, they spend more and more and more money, that’s this yellow line to do this very antiquated process. And less and less and less drugs are being coming out of this pipeline.
Because this pipeline is frankly very primitive. It doesn’t work and more of the drugs coming out have side effects, even the FDA is not allowing them. So you have to consider why pharma companies are interested in vaccines.
Well, vaccines are considered a biologic, they’re not a drug, they don’t need to go through that same regulatory process. And by the way, you cannot sue pharma companies in court, you have to go to a health and human services court. And more importantly, they don’t have to go through all those regulations.
So pharma companies, that’s why they’re moving towards vaccines, less less testing they have to do and it’s a very, very different model. So let’s go back here. So as we see here, and so this was one of the reasons that I was interested inside itself, because I saw this whole process was really dumb.
The other thing is in 2003, when the Genome Project ended right here, we thought we had 100,000 genes. The whole theory of biology was if you have this gene, you’re gonna get this disease. Let’s say you have the gene for osteoarthritis, you’re gonna get bad joints.
Well, it turns out that we only have 20,000 genes, the same number of genes as a worm. So what does that mean? That means that you are not your genes. So biology for since 1950, since the discovery of DNA was saying, if you have this gene, you’re gonna get this disease.
And so since 1950, all the way to 2003, we gotta find that gene, what’s the gene? What’s the gene, and the idea was, well, we must have more genes and a worm were more complex, a worm at 20,000 genes, the irony, the revolution in biology 2003 occurred, because we found that we, as human beings only have 20,000 genes, the same number of genes as a worm. So this meant it’s not the genes that are the issue that we had to take what’s called a systems approach. You see, the reductionist approach is always like the blind man, just thinking this one part is everything on climate change, oh, my god, co2 is everything, not looking at the whole system.
So that same revolution occurred in biology? All right. So what occurred in biology that was fascinating here was a recognition that genes are not of makes us who we are, it is actually the fact that if you want to understand the whole human being, you have to go from genes to proteins to cells and tissues, you have to go across this entire span of different genes and proteins. In fact, you have to start looking at the cell not as a nucleus as a command and control center, but a whole interaction, an ensemble of chemical reactions, a system, so it’s not so the genes may be a small part of what you eat.
So what you eat may turn on certain genes, what you think what exercises you do, and that’s what we’re finding, it’s not a one way street. It’s actually two ways, genes and proteins and all these things, highly, highly interactive. So again, to somebody, the old models have this gene, I’m gonna get this disease, I’m screwed.
No, the new model of biology is let’s understand the molecular reactions. Well, when that’s in 2003, is when I returned back to MIT, my old adviser Forbes, do we sit Shiva, you create an email, you’re into computing, you’ve done all this great stuff, you’ve always loved medicine. Now there’s a systems approach, the field could use your skills to imagine creating a technology that can mathematically model all of those chemical reactions on the computer, we can completely revolutionize medicine.
So that’s what I did between 2003 to 2007, I saw here are these little molecular pathways, I can make the mathematical models. But in order to do something this complicated, I would have to go be able to integrate many, many systems of models. And that was a declaration of CytoSolve, where it allowed us I created a whole technology where I could create small models and integrate them on the fly, which means we can handle complexity.
And that has resulted over the last 16 years in the development of CytoSolve as a company, but we are actually enabling truth, freedom in health, because we’re doing all of this research in a decentralized, inclusive, transparent, open, personalized, systems based way where we’re mining the literature, we’re creating models, and we share We’re not into like hiding everything when we find large sites. And when we build our own products, obviously, we it’s a business thing. But when we find knowledge, we share it with everyone, I’ll show you an example we’ve done right here in joints out so and by the way, I published a very important paper back in 2011, really showing all this but fundamentally CytoSolve.
It’s not just taking a single compound by pharmaceutical companies, I can take complex compounds that occur in nature, run it through CytoSolve, long before we do test tube testing, by mining all the work. By the way, this is how we do this is how we build airplanes, we don’t just throw a pilot and say, oh, let’s kill them or maybe dies or not. We do everything on the computer, then we go do different designs.
This is the future of medicine. And that’s what CytoSolve is. All right.
So when we looked at joint health, what we did was we went through nearly 22,000 papers across the entire world. And we were funded by the Arthritis Foundation, as well as the University Health Network of Canada, I was giving a talk to a bunch of arthritis people and they said, Wow, you have something pretty incredible, we want to support you. And what we did was we took us back two to three years, we mapped out every molecular reaction involved in joint health, then we put all of that together and one of the biggest maps, it’s like imagine charting that galaxy.
And then we have made that accessible to all of you, if we didn’t put it in our own little cubbyhole, we’ve created that we can share that with everyone, I’m going to share that with you. So we have the first molecular systems map of osteoarthritis. And with that map, you can then start understanding, you can start understanding joint health.
Let me go back to this. And those of you can access this. So if you go to this is the paper that we wrote.
And I’m going to log right into this. And let me go right here, I have to switch over here, we stopped the screen. I’m going to now I just have to bring up the site, it’s all.
com. So I’m going to now take you into CytoSolve we have an open science, I’m a big proponent of citizen science, imagine I could share with you knowledge you can contribute science can evolve much faster rather than relying on five colleges will get funded by Fauci and other people with a lot of political agenda that we’re finding. So if we go here, let me share the screen here.
And I’m gonna go right here. So I’m gonna go to right here, so everyone should be able to see this and I’m gonna go right here. This is CytoSolve.
com. And here is so we have a whole research paper on it. By the way, if you want to go here and read there’s a whole research paper It’s free.
You can download it. If we had published this in one of those journals that would cost you hundreds of bucks behind a paywall, you can actually go read about this entire analysis that we did three years worth of effort that we did, I wanted to let you know that we use the technology here. We’ve helped many, many companies over the last 16 years, a lot of smart, innovative companies.
But we decided with all the mathematical models we’ve created, why don’t we try to use this to compute the best product we could think of from the science out there for reducing pain and inflammation, pain and discomfort, and that resulted in us Creating m v 25. Using CytoSolve we’re going to have more products that are going to be coming. Let me just show you what MV 25 is about for those of you who haven’t heard about it, but this is using psilocybin a beneficial way not to just do research but find combination therapies.
I am firebrand, my hands would cramp up so that I couldn’t pull cards or knit or crochet. Me would go like that. Not have to use this when I played cards with my grandkids and I started taking that MV 25 After betta was able to hold cards in my hand.
Very, very little cramping hardly at all anymore. MV 25 Hi, my name is Sanjay, I’m a Taekwondo instructor, I tore my ACL during Taekwondo, I had a lot of pain and limited mobility. I’ve been taking the MV 25 for about six months now after the first week, I noticed the big difference after the second week, almost literally no pain.
My name is Jeremy and I suffered from a lower back problem hurt my back at work years ago. And I can go to the chiropractor and do all kinds of different things. And nothing seems to help.
And I decided to try MV 25, I didn’t notice a difference immediately. But within a few days of pain went away and it stayed away. I’ve continued to take it.
And even when I do things that I shouldn’t do, it seems to go away a lot quicker than I ever did before. It’s clean foods certified, it’s made in the US, if you go to bat shiva.com Right on the shop, you’ll click there.
Or you can go right to MB 25 dot like either way. And then from there, you can click on the bottle and you can order if you buy six bottles, you get six bottles for free, please take advantage of it because first of all, it’s gonna help you it’s going to help our movement and it really supports the fact that we want to take science based approaches to natural products, right? So when we talk about glucosamine, everything I’ve just shared with you is the same approach we take to understanding glucosamine we take a systems approach we don’t just take Well, I just want to understand it in some random way. We take a systems approach to understanding it using the technology of CytoSolve, which we’re fortunate to have.
So someone says they lost audio. Is that true? Can everyone hear All right? Great. No one can hear audio, just checking it good.
So someone said Okay, so let’s look at what is glucosamine. So here, I always begin with looking at the molecular structure of glucosamine. And what do you see here? First of all, as I mentioned over nearly 30,000 research articles, but this is what glucosamine looks like it’s got a bunch of you see this O H OHOH is a call hydroxyl groups and it’s got this amino group so this is what’s called gluco to me, so part of the two structural polysaccharides Chiverton which forms the exoskeletons of insects fungi cell wall shells and shellfish and China sand which forms the shells and shellfish this molecule when connected forms the exoskeleton of insects.
So it’s an infrastructure it supports infrastructure, it’s click clinically shown to have symptomatic relief for joint pain and there’s no known supplement drug interactions, so it’s relatively safe from the research we’re sharing with you today. The glucose main sources where does glucosamine come from? Well, you can see comes from shellfish shells and liminal bones, bone marrow, fungi, bone marrow, if you study some of the traditional systems of medicine, you’ll find that in just a quick aside, but the marrow of bones is called the gene of Chinese medicine is where they say that power comes from. So in many cultures, they would crack bones, and they do bone soup.
But the marrows are literally were even in your own bodies where the stem cells are created for red blood cells. So bone marrow is a very, very powerful nutrient, but as you can see here, it’s derived from bone marrow. The other piece here is what are the biological effects where there are three biological effects here anti inflammatory, antioxidant, and it’s Khandro protected? What does that mean? Khandro sites are cartilage we’re going to shortly learn that there are different cells in the in joints.
So if you look at your knee joint, there are different kinds of cells there. There’s synovial, there’s fat pad meniscus, but there’s also cartilage is composed of what’s called Khandro. Sites, just like red blood cells is a type of cell.
It’s called Condor sites. So the Condor sites as you age as let’s say you do weights or you push hard you can tend to hurt your cartilage. Well, glucosamine is Khandro protected, it’s Khandro protected, which means it protects cartilage.
The health benefits are AR from the analysis is it reduces cartilage degradation. It improves non arthritic knee injury improves range of motion or reduces pains and joints improves the skin health and it’s accelerates wound healing for better skin hydration and reduce wrinkles. There’s a lot of research out of those 30,000 papers pointing to all of these different benefits.
Alright, cartilage degradation reduction, improving non arthritic knee injury, improving range of motion reducing pain and joints improve skin health. And the other subset of this it accelerates wound healing better skin hydration, which means it helps your body store water and reduces wrinkles. But over here, you can literally use the visual graph.
It’s like Google Maps, and you can literally this is the knee joint and we’re looking at all the different tissues in the knee, I can go look at one of the tissues like synovial, it gives me all the cells I can perhaps look at cartilage, which is composed of as I mentioned, Congress sites and within Congress sites, I can go look at different live games, what are like against ligands are the particles that can land on the cells and cause a molecular reaction here are different kinds of ligands. There’s growth factors, your different kinds of growth factors. But that’s not the only cause there’s things called cytokines, which you’ve heard of these are the ones that can create an inflammation in your body.
Here are different kinds of cytokines that land on the Condor sites on cartilage. One of them is il one beta, which I’ve shared. So you can look at all of these green molecules.
If you have il one beta in your body, all these other green molecules will get upregulated, which means they’ll start showing up and the red ones are the ones that will start going down. So you can see there’s tons and tons of all of these molecules that start showing up. So for example, here’s TNF alpha, TNF alpha has an inflammatory effect, right? And then you can start seeing the ways that works.
And I’ll get back to that you can go back to you can understand, for example, Iowan beta, and the one I want to talk about cartilage regeneration is MMP 13. Now, what can you do with this tool as you can so let’s look at the catabolic effect. Well, what’s the catabolic effect, the catabolic effect is the ability to that’s where it regenerates cartilage.
And here, you can actually look at the molecular pathways. So I hope everyone’s tracking here, what we’re doing here, which CytoSolve is we have a incredible approach that we can map all the molecular reactions across all those 30 20,000 papers, and we’re looking at all the ways things interact to produce, in this case, cartilage degeneration, and then we can look closely almost like a microscope. And right here, I’m going at looking at the fact that il one beta causes MMP 13.
But look all these other nutrients that get in the way. So ginseng, for example, if I click on this, you’ll find ginseng is something that stops and here’s the paper, here’s a research or there’s someone on YouTube or Google or Twitter says, Oh, I don’t believe well, here’s the actual research. Here’s a Kandra protective effects of ginseng on osteoarthritis and similar you can use this find out well what happens if I take what’s this one here? Whoa, Jeanette, okay, there’s another thing that is volgende enrich Richardson.
Vojin is another nutrient that you can find. You can also look at, for example, resveratrol, which comes in the skin of red grapes. But this is a very powerful tool that actually helps you explore all those pathways.
So that’s what we were able to do with CytoSolve. We were able to map all that out. Now when it comes to understanding glucose means effect we took out of all those molecular mechanisms, we started looking at what were the real things that can affect joint health.
All right, and what we’re going to share with you here is those particular pathways, so I just walked you through that. So here are the four mechanisms that are involved in joint inflammation, oxidative stress, cartilage degeneration, cartilage regeneration, we went over this we talked about how MSM really helps oxidative stress reduction. We talked about a hyaluronic acid yesterday, help improves cartilage regeneration.
We talked about how copywriting can lowered cartilage degeneration, we talked about this, but what is glucosamine do and by the way, these are all the pathways like inflammation has a whole bunch of pathways, oxidative stress has hit a whole bunch of molecular reactions, cartilage as its reactions and cartilage degeneration. So all of these mechanisms were able to analyze bioinformatics, but also computationally, and by the way, each one of these mechanisms has a particular variable. So inflammation we want to bring down oxidative stress we want to bring down cartilage during generation we want to bring down cartilage regeneration we want to bring up that’s denoted by the green.
So each of these phenomenon have different biological markers or biological components which are indicative of that. So PG two is indicative of inflammation. Reactive oxygen species is indicative of oxidative stress.
MMP 13 is indicative of cartilage degeneration, cartilage regeneration, collagen to we spoke about yesterday how hyaluronic acid increases. So when we look at glucose means effect on joint health. What this is what we’re finding from the bio informatics analysis.
So just to be clear, I want to just make this clear. CytoSolve does two kinds of analysis. One is looking at all the papers and seeing what they say and then one is we extract out the molecular pathways mathematically model that’s called The computational so the first part now is we’re gonna look at the bioinformatics analysis of how glucosamine works.
Remember, we’re taking a systems approach, glucosamine has a multiplicative multiplicative effect doesn’t just do one thing. It’s not like the blindman. In nature nature, it does many things.
So you have to be careful when you start playing with nature because you can have offsetting effects. So here we’re seeing here is that glucose, glucose amine here, the glucosamine that affects three of the joint health mechanisms, oxidative stress, inflammation, cartilage degeneration, glucosamine lowers oxidative stress, you see that that’s what the hammer means the red hammer, so you should this is in biology, lowers oxidative stress, it lowers inflammation, it lowers cartilage degeneration by doing this, it makes sure you don’t get joint disease to get joint health actually. So these are the three mechanisms that we’re going to explore how does glucosamine reduce PG two, how does it reduce RLS? And how does it reduce MMP 13.
So just to be clear, the research shows a glucosamine doesn’t help you increase cartilage regeneration, it looks right now from the bioinformatics it helps you lower this helps you lower this let’s go a little bit deeper. How does it help you look at let’s say we’re gonna look at first we’re gonna look at oxidative stress. Actually, let me go to inflammation.
First, we’ll come back to this. So how does glucosamine affect inflammation? Well, remember that il one beta that I showed you that cytokine is what creates inflammation, and it does have two ways glucosamine il one beta uses this pathway NF Kappa Beta. So il one beta pro inflammatory cytokines such as activate p 38.
Map que right here on the left, and it also activates NF Kappa Beta. You don’t want these in your body, meaning at high levels, and this will produce il one eight and this produces NF Kappa Beta which produces Cox two which gives PG Joe by the way, these are also to be clear, these are pathways related to cancer, high inflammation, these variables are high in your body, so you want to be aware of that. So il one beta produces this this gives you inflammation NF Kappa Beta Cox two PG two that gives you inflammation and you get joint disease Okay, okay.
What does glucose glucose mean? What comes in and blocks il one beta so it stops expression of p 38. And it stops the expression of NF Kappa Beta and aisle eight and PG two both of these Cox two is an enzyme they increase inflammation so boom, glucosamine stops it right up front, it stops Iowan beta this is how it reduces inflammation. Second thing is how does glucosamine reduce oxidative stress? So what is oxidative stress? Well, if you have oxidative stress is basically your body’s rusting, aging and oxidative stress is exhibited by a molecule called RLS.
Reactive oxygen species. There’s a variety of reactive oxygen species, not only one molecule, there’s a whole bunch of but what we can see here what glucosamine does, it’s there are two things which create oxidative or reactive oxygen species. So under disease conditions, RLS is produced from NADPH oxidase that’s an enzyme that produces it and whenever you see a sc, that’s an enzyme and electron transport chain and mitochondria.
So your mitochondria are doing metabolism. Well, one of the aspects of running your engine just like you get smoke coming out right out of the back or exhaust exhaust is reactive oxygen species. So excess RLS if it’s not clean up will cause oxidative stress and chronic oxidative stress leads to joint disease.
So if you don’t have proper cleanup mechanisms in your body, all right, you’re gonna get oxidative stress, what is glucosamine do it bank stops RLS so it stops from you getting joint disease. All right. So that’s the second way that glucosamine works right here because to me, the stopping the production of reactive oxygen species, so we’ve covered inflammation, oxidative stress, and let’s finish up with cartilage degeneration.
We have seen from the research that we’ve done across all those papers, the inflammatory cytokine Island beta, this is a hallmark of joint disease. Let me explain what that means. If you have Iowan beta in your body, it’s like the indicator of joint disease.
All right, so it’s a great way that So, if you go to your doctor and you can talk to him about Iowan beta, but Iowan beta, you can maybe even educate them Iowan beta is a big indicator of joint disease. So, what you’re seeing is Iowan beta gives rise to this P 38 MMP K, but P 38. Just like it produced inflammation also produced MMP 13 and MMP 13 is what eats away your cartilage this causes a catabolic effect.
The good news is there are nutrients glucosamine is one of them, which stops us Iowan beta going to p 38. And it and remember, MMP 13 degrades that collagen. That’s the way this works in cartilage thereby promoting joint disease via loss of cartilage and glucosamine inhibits this activation leading to downregulation of MMP 13.
So I hope that helps. So right now, what we’re covering to everyone joining new is we’ve just covered using CytoSolve. But we looked at all the literature and finding that out of four things that affect joint health three of them.
Bioinformatics shows that the literature shows that glucosamine can lower inflammation, it can lower oxidative stress is and can lower cartilage degradation. Now we’re going to move to using CytoSolve other capability where we do all the rocket science of modeling all the molecular reactions of these mechanisms. So right now we believe that glucosamine affects these three things.
But the bioinformatics analysis we’ve done points to us that glucosamine affects inflammation, oxidative stress and cartilage degradation, which means it lowers it. Now we’re going to do the computational analysis. Remember PG E two on the y axis is a marker of inflammation.
So the literature said, Oh, PG to lower inflammation. So what we did is we said, Let’s give 250 milligrams to 500 milligrams over two days, and we’re doing this on the computer. Remember, we’re not killing animals.
Here, we’re not doing dissections, we’re modeling those molecular pathways of inflammation, and we’re putting in glucosamine, and we’re seeing what it does. And we’re very conservative here. So what we’re saying is if you have 14, so you have a certain amount of not a moral molars of PG two here, you can see as we dose as we give greater dosage of PG two, it doesn’t really seem to have that much effect on inflammation.
So while the literature says that our computational analysis says glucose, glucosamine did not have a lot of effect on reducing inflammation, when we looked at oxidative stress, we see something very different. So when we give that same dosage zero to 500 milligrams, and we’re dosing it over two days under controlled conditions, who coasted me it looks like it’s has a very powerful effect from 160 nanometers, we’re down to almost less than 150, let’s say 145, or something like that. So it’s almost as a 20% reduction by 20%, in bringing down our 20% and bringing down the level of oxidative stress, and that’s only with about 20 250 to 500 milligrams over two days, we wanted to be conservative, that’s what we see here.
So our research says glucosamine is a very powerful effect. Again, I hope everyone’s tracking, we did the bioinformatics research, but we don’t just want to believe the literature because we can model the molecular reactions, we can do our own powerful research here. Next, we’re looking at the effect of glucosamine on cartilage degradation.
Again, we did the same thing. So here we have a very high level of cartilage degradation 457 Six, and we see as we dosage from 50 100 200 milligrams and so on over two days, it has also a effect from 456 down to 450. So it’s not as profound as what happens with oxidative stress, it’s moderate.
So we found it has low to moderate effect and cartilage degradation. So in summary, it affects all these three but we did a little if you can see this we have we do these little heat maps. And you can see it has really no effect on inflammation.
It has no effect on cartilage regeneration has some moderate effect and car cartilage degradation, but it really helps reduce the oxidative stress. That’s what side hustles Reacher’s research shows. So if you were to look at this, it doesn’t do much for inflammation or analysis.
It does a lot for oxidative stress and for cartilage degeneration. So I hope that teaches you right, and by the way, CytoSolve we’re constantly adding new research. It’s like a It’s like your iOS operating system.
So in the joint health space, we get new literature, we rerun molecular pathways. Alright, everyone, have a good weekend. Thank you be well
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